Application of Oligonucleotide Activation to Restriction Endonuclease NarI
نویسندگان
چکیده
منابع مشابه
Application of oligonucleotide activation to restriction endonuclease NarI.
Restriction endonuclease NarI cleaves DNA using a two-site mechanism, placing it in the Type IIe class of restriction endonucleases. Although these enzymes have very useful recognition sequences, the two-site mechanism limits the practical application. Site preferences often cause incomplete substrate digestion. Oligonucleotide activation of NarI eliminates incomplete digestions, making it poss...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 1997
ISSN: 0736-6205,1940-9818
DOI: 10.2144/97226pf01